Gent, L and Chiappetta, ME and Hesketh, S and Palmowski, P and Porter, A and Bonicelli, Andrea and Schwalbe, EC and Procopio, Noemi Bone proteomics methods optimisation for forensic investigations. [DataSet]

Description

The application of human bone in forensic proteomics is an expanding novel purpose in the aim of successfully quantifying biological estimations used in medico-legal investigations with greater accuracy. In this project, different extraction protocols were tested on n=30 human bone samples, including S-Trap technology with two different lysis buffer, and ZipTips. Additionally, a comparison was made between data-dependent acquisition (DDA) and data-independent acquisition (DIA) mode. Results showed less missing data using S-Traps instead of the more routine reverse-phase media tips (ZipTip). The type of lysis buffer when using S-Traps does not impact largely the analysis conducted in forensic proteomic workflows. Lastly, it was found that when open-source software is used for data processing for both DDA and DIA modes, DIA has the upper advantage in terms of acquiring a larger number of proteins due to its greater sensitivity to those with a lower abundance.

Research / Data Type:	Dataset
Depositing User:	CLoK admin
Date Deposited:	09 Jul 2024 11:19
Revision:	7
URI:	https://uclandata.uclan.ac.uk/id/eprint/459
Additional details Additional details Creators: Creators Email ORCID Gent, L UNSPECIFIED UNSPECIFIED Chiappetta, ME UNSPECIFIED UNSPECIFIED Hesketh, S UNSPECIFIED UNSPECIFIED Palmowski, P UNSPECIFIED UNSPECIFIED Porter, A UNSPECIFIED UNSPECIFIED Bonicelli, Andrea ABonicelli@uclan.ac.uk UNSPECIFIED Schwalbe, EC UNSPECIFIED UNSPECIFIED Procopio, Noemi NProcopio@uclan.ac.uk UNSPECIFIED Schools: School of Law and Policing* > Law* Contact email address: rdmsupport@uclan.ac.uk Last Modified: 09 Jul 2024 11:24

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Related Resources

Bone proteomics ... investigations Bone proteomics ... investigations Bone proteomics ... investigations Resource Title: Bone proteomics methods optimisation for forensic investigations Resource Type: This dataset stored elsewhere Resource Identifier: 10.25345/C5QN5ZP1H Identifier Type: DOI URL: https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?accession=MSV000093803

Bone Proteomics ... Investigations Bone Proteomics ... Investigations Bone Proteomics ... Investigations Resource Title: Bone Proteomics Method Optimization for Forensic Investigations Resource Type: Publication Resource Identifier: 10.1021/acs.jproteome.4c00151 Identifier Type: DOI URL: https://doi.org/10.1021/acs.jproteome.4c00151