Bonicelli, Andrea and Taylor, George and Procopio, Noemi (2024) Extraction and Untargeted Analysis of Metabolome from Undemineralised Cortical Bone Matrix for Forensic Application. [DataSet]

Description

Untargeted metabolomics has become the gold standard for the profiling of low-molecular-weight compounds. Recently, metabolomics has shown great potential in forensic science in the field of toxicology and postmortem interval estimation. The current study aims to evaluate three extraction protocol and four liquid chromatography coupled with mass spectrometry assays that could offer a valuable tool to identify biomarkers for PMI estimation. One fragment for anterior human skeletal tibia from a 82 years old male individual belonging to the Forensic Anthropology Center - Texas State University collection was powdered and extracted in five replicates to be extracted according to a the biphasic chloroform/methanol/water protocol and two single phase protocols based on methanol/water and methanol/acetonitrile/water. Formal analysis was carried out ThermoFisher Ultimate 3000 HPLC in hydrophilic interaction (HILIC) and reverse phase (RP) liquid chromatography coupled with SCIEX 6600 TripleTOF Q-TOF mass spectrometer.

Research / Data Type:	Collection - various types
Research Centres:	Field Archaeology and Forensic Taphonomy
Depositing User:	Christopher Waddington
Date Deposited:	08 Jan 2025 14:07
Revision:	20
URI:	https://uclandata.uclan.ac.uk/id/eprint/506
Additional details Additional details Creators: Creators Email ORCID Bonicelli, Andrea ABonicelli@uclan.ac.uk orcid.org/0000-0002-9518-584X Taylor, George UNSPECIFIED UNSPECIFIED Procopio, Noemi NProcopio@uclan.ac.uk orcid.org/0000-0002-7461-7586 Schools: School of Law and Policing* > Policing, Criminology, Forensics and Archaeology* Date: 17 March 2024 Date type: Publication Projects: Grant Number Funder Name Funder ID MR/S032878/1 UKRI Future Leaders Fellowship UNSPECIFIED Status: Published Contact email address: rdmsupport@uclan.ac.uk Last Modified: 08 Jan 2025 14:07

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Extraction and ... cal Bone Matrix Extraction and ... cal Bone Matrix Extraction and ... cal Bone Matrix Resource Title: Extraction and Untargeted Analysis of Metabolome from Undemineralised Cortical Bone Matrix Resource Type: Publication CLoK URL: https://clok.uclan.ac.uk/52329/

Extraction and ... sic Application Extraction and ... sic Application Extraction and ... sic Application Resource Title: Extraction and Untargeted Analysis of Metabolome from Undemineralised Cortical Bone Matrix for Forensic Application Resource Type: This dataset stored elsewhere URL: https://doi.org/10.21228/M8DX4F